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Glotzer, Michael A.
One or more keywords matched the following properties of Glotzer, Michael A.
PropertyValue
keywords Cell Polarity
keywords Cytokinesis
keywords Centralspindlin
overview The Glotzer lab focuses on questions related to cell organization. How do cells coordinate the position of the contractile ring with the position of the spindle? By what mechanisms are cortical domains that mediate cell polarization assembled and maintained? How do cells regulate cortical contractility during developmental morphogenesis? To answer these questions, we use the nematode C. elegans, cultured human cells, budding yeast, and Drosophila as model systems and we combine forward and reverse genetics, biochemistry, and live cell imaging. Through these approaches we have discovered and extensively characterized the centralspindlin complex, a multifunctional protein complex that regulates essentially every step of cytokinesis. We have pioneered the use of optogenetics to dissect spatiotemporally regulated processes. Using these approaches, we have demonstrated that RhoA activation is sufficient to induce cleavage furrows irrespective of the position of the spindle or the stage of the cell cycle. Likewise, we obtained direct evidence that positive feedback is active during yeast cell polarization. By combining optogenetics with "conventional" genetics and live cell imaging we can directly test models for how cells endow well defined cortical regions with distinct properties that are required for complex cellular events, like cell division, cell polarization, and tissue scale morphogenetic events, such as gastrulation.
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Concept Actins
Concept Adenylyl Imidodiphosphate
Concept Adenosine Diphosphate
Concept Anaphase
Concept Adenosine Triphosphatases
Concept Adenosine Triphosphate
Concept Actomyosin
Concept Catalysis
Concept Chromatography, DEAE-Cellulose
Concept Epitopes
Concept Base Sequence
Concept Autoantigens
Concept Bacterial Proteins
Concept Binding Sites
Concept Cell Membrane
Concept Cell Division
Concept Child
Concept Cells
Concept Eukaryotic Cells
Concept Cell Cycle
Concept Chromosome Mapping
Concept Fungal Proteins
Concept Cold Temperature
Concept Chromatids
Concept Chromosomal Proteins, Non-Histone
Concept Culture Media
Concept Contractile Proteins
Concept DNA Transposable Elements
Concept DNA-Binding Proteins
Concept DNA, Mitochondrial
Concept Cytoskeleton
Concept Dose-Response Relationship, Radiation
Concept Enzyme Inhibitors
Concept Epithelial Cells
Concept Cell Biology
Concept Microtubule-Associated Proteins
Concept Terminology as Topic
Concept Guanosine Triphosphate
Concept Hypotonic Solutions
Concept Immune Sera
Concept Escherichia coli
Concept Membrane Glycoproteins
Concept Protamine Kinase
Concept Mathematical Computing
Concept Membrane Proteins
Concept Microfilament Proteins
Concept Models, Molecular
Concept Actin Cytoskeleton
Concept Mitosis
Concept Neurospora crassa
Concept Organometallic Compounds
Concept Molecular Sequence Data
Concept Molecular Weight
Concept Mice, Inbred C57BL
Concept HeLa Cells
Concept Ion Channels
Concept Endopeptidases
Concept Metaphase
Concept Phosphoproteins
Concept Plant Proteins
Concept Phosphoprotein Phosphatases
Concept Plasmids
Concept Proto-Oncogene Proteins
Concept Proteins
Concept Protein Kinases
Concept Radiation Dosage
Concept RNA
Concept Telophase
Concept Histones
Concept Time Factors
Concept Caenorhabditis elegans
Concept Sequence Homology, Amino Acid
Concept Trans-Activators
Concept Helminth Proteins
Concept Genes, cdc
Concept Insect Proteins
Concept Chromosome Segregation
Concept Cryoelectron Microscopy
Concept Guanine Nucleotide Exchange Factors
Concept rho GTP-Binding Proteins
Concept Protein Structure, Quaternary
Concept Mitogen-Activated Protein Kinases
Concept Protein Subunits
Concept Myosin Type II
Concept Meiosis
Concept Maturation-Promoting Factor
Concept Alternative Splicing
Concept Protein Structure, Tertiary
Concept Myosins
Concept Ubiquitin Thiolesterase
Concept Ubiquitin-Protein Ligase Complexes
Concept RNA-Binding Proteins
Concept Receptors, Cytoplasmic and Nuclear
Concept Qa-SNARE Proteins
Concept Apoptosis Regulatory Proteins
Concept Centrosome
Concept Adhesins, Bacterial
Concept Drosophila Proteins
Concept Protein Tyrosine Phosphatases
Concept GTP Phosphohydrolases
Concept rab GTP-Binding Proteins
Concept Mitochondrial Proteins
Concept Ubiquitin-Conjugating Enzymes
Concept Green Fluorescent Proteins
Concept NIH 3T3 Cells
Concept Actin-Related Protein 2
Concept COS Cells
Concept Cyclin B
Concept Cell Cycle Proteins
Concept rhoA GTP-Binding Protein
Concept rac GTP-Binding Proteins
Concept Schizosaccharomyces pombe Proteins
Concept Radiotherapy, Conformal
Concept Phosphorus Radioisotopes
Concept Saccharomyces cerevisiae Proteins
Concept Pteridines
Concept Recombinant Proteins
Concept RNA, Double-Stranded
Concept Saccharomyces cerevisiae
Concept Serine
Concept RNA, Messenger
Concept Mechanotransduction, Cellular
Concept Ubiquitin-Protein Ligases
Concept Multiprotein Complexes
Concept HEK293 Cells
Concept Septins
Concept Substrate Specificity
Concept Adaptor Proteins, Signal Transducing
Concept CDC2 Protein Kinase
Concept Cyclins
Concept Thiolester Hydrolases
Concept Tubulin
Concept Ubiquitins
Concept Cell Polarity
Concept Proliferating Cell Nuclear Antigen
Concept GTP-Binding Proteins
Concept cdc42 GTP-Binding Protein
Concept Myosin Type V
Concept Ubiquitin
Concept Caenorhabditis elegans Proteins
Concept Molecular Motor Proteins
Concept Monomeric GTP-Binding Proteins
Concept GTPase-Activating Proteins
Concept cdc42 GTP-Binding Protein, Saccharomyces cerevisiae
Concept Structural Homology, Protein
Concept I-kappa B Kinase
Concept Smad2 Protein
Concept Zyxin
Concept RNA, Small Interfering
Concept Cell Line, Tumor
Concept Intracellular Signaling Peptides and Proteins
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Concept Topoisomerase II Inhibitors
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Concept Plant Cells
Academic Article Cytokinesis: regulated by destruction.
Academic Article Centrosome separation and central spindle assembly act in redundant pathways that regulate microtubule density and trigger cleavage furrow formation.
Academic Article Kleisins: a superfamily of bacterial and eukaryotic SMC protein partners.
Academic Article Central spindle assembly and cytokinesis require a kinesin-like protein/RhoGAP complex with microtubule bundling activity.
Academic Article A ubiquitin C-terminal hydrolase is required to maintain osmotic balance and execute actin-dependent processes in the early C. elegans embryo.
Academic Article The aurora B kinase AIR-2 regulates kinetochores during mitosis and is required for separation of homologous Chromosomes during meiosis.
Academic Article GTP binding induces filament assembly of a recombinant septin.
Academic Article Mammalian septins nomenclature.
Academic Article Cleavage furrow positioning.
Academic Article Comparative analysis of cytokinesis in budding yeast, fission yeast and animal cells.
Academic Article Cytokinesis: progress on all fronts.
Academic Article Cytokinesis: a logical GAP.
Academic Article Structural analysis of the ZEN-4/CeMKLP1 motor domain and its interaction with microtubules.
Academic Article Cell cycle regulation of central spindle assembly.
Academic Article Polo-like kinase 1 triggers the initiation of cytokinesis in human cells by promoting recruitment of the RhoGEF Ect2 to the central spindle.
Academic Article Developmental regulation of central spindle assembly and cytokinesis during vertebrate embryogenesis.
Academic Article Control of cortical contractility during cytokinesis.
Academic Article Cytokinesis: GAP gap.
Academic Article Sequential Cyk-4 binding to ECT2 and FIP3 regulates cleavage furrow ingression and abscission during cytokinesis.
Academic Article Prospects for microbeam radiation therapy of brain tumours in children.
Academic Article An ECT2-centralspindlin complex regulates the localization and function of RhoA.
Academic Article Regulation of cortical contractility and spindle positioning by the protein phosphatase 6 PPH-6 in one-cell stage C. elegans embryos.
Academic Article Clustering of centralspindlin is essential for its accumulation to the central spindle and the midbody.
Academic Article Controlling cytokinesis through promiscuous phosphorylation outside BARs.
Academic Article Cytokinesis: integrating signaling, the cytoskeleton, and membranes to create new daughter cells.
Academic Article Anillin promotes astral microtubule-directed cortical myosin polarization.
Academic Article Single cells (put a ring on it).
Academic Article RhoA activation during polarization and cytokinesis of the early Caenorhabditis elegans embryo is differentially dependent on NOP-1 and CYK-4.
Academic Article TULIPs: tunable, light-controlled interacting protein tags for cell biology.
Academic Article Centralspindlin: at the heart of cytokinesis.
Academic Article The RhoGAP domain of CYK-4 has an essential role in RhoA activation.
Academic Article The molecular requirements for cytokinesis.
Academic Article Phosphorylation of ZEN-4/MKLP1 by aurora B regulates completion of cytokinesis.
Academic Article Cytokinesis: welcome to the Rho zone.
Academic Article Cooperative assembly of CYK-4/MgcRacGAP and ZEN-4/MKLP1 to form the centralspindlin complex.
Academic Article Polo-like kinase 1 directs assembly of the HsCyk-4 RhoGAP/Ect2 RhoGEF complex to initiate cleavage furrow formation.
Academic Article The CeCDC-14 phosphatase is required for cytokinesis in the Caenorhabditis elegans embryo.
Academic Article CSC-1: a subunit of the Aurora B kinase complex that binds to the survivin-like protein BIR-1 and the incenp-like protein ICP-1.
Academic Article Astral signals spatially bias cortical myosin recruitment to break symmetry and promote cytokinesis.
Academic Article Anillin is a scaffold protein that links RhoA, actin, and myosin during cytokinesis.
Academic Article The 3Ms of central spindle assembly: microtubules, motors and MAPs.
Academic Article Mitochondrial uncoupling protein from mouse brown fat. Molecular cloning, genetic mapping, and mRNA expression.
Academic Article Binding of the CYK-4 subunit of the centralspindlin complex induces a large scale conformational change in the kinesin subunit.
Academic Article Cytokinesis: centralspindlin moonlights as a membrane anchor.
Academic Article Aurora B kinase promotes cytokinesis by inducing centralspindlin oligomers that associate with the plasma membrane.
Academic Article The RhoGAP activity of CYK-4/MgcRacGAP functions non-canonically by promoting RhoA activation during cytokinesis.
Academic Article Cytokinesis: Placing the Furrow in Context.
Academic Article Local RhoA activation induces cytokinetic furrows independent of spindle position and cell cycle stage.
Academic Article Optical Control of Peroxisomal Trafficking.
Academic Article Regulation of cortical contractility and spindle positioning by the protein phosphatase 6 PPH-6 in one-cell stage C. elegans embryos.
Academic Article Cytokinesis in Metazoa and Fungi.
Academic Article Optogenetic control of RhoA reveals zyxin-mediated elasticity of stress fibres.
Academic Article Cell cycle entry triggers a switch between two modes of Cdc42 activation during yeast polarization.
Academic Article Spatiotemporal Regulation of RhoA during Cytokinesis.
Grant Spatial and temporal control of cell behavior with a genetically-encoded photoswi
Grant Spatial and temporal control of Rho family GTPases
Grant Positioning the Plane of Cell Division During Cytokinesis
Grant Molecular Dissection of Cytokinesis
Academic Article Animal cell cytokinesis.
Academic Article Cytokinesis.
Academic Article Mitosis: don't get mad, get even.
Academic Article Depletion of syntaxins in the early Caenorhabditis elegans embryo reveals a role for membrane fusion events in cytokinesis.
Academic Article Chromosome segregation: Samurai separation of Siamese sisters.
Academic Article CDK1 inactivation regulates anaphase spindle dynamics and cytokinesis in vivo.
Academic Article Cytoplasmic flows localize injected oskar RNA in Drosophila oocytes.
Academic Article Mutagenic analysis of the destruction signal of mitotic cyclins and structural characterization of ubiquitinated intermediates.
Academic Article Cyclin is a component of maturation-promoting factor from Xenopus.
Academic Article Separase is required for chromosome segregation during meiosis I in Caenorhabditis elegans.
Academic Article The mechanism and control of cytokinesis.
Academic Article Cell polarity. The importance of being polar.
Academic Article Cell cycle. The only way out of mitosis.
Academic Article Cyclin is degraded by the ubiquitin pathway.
Academic Article Cyclin activation of p34cdc2.
Academic Article Incenp and an aurora-like kinase form a complex essential for chromosome segregation and efficient completion of cytokinesis.
Academic Article CYK-4: A Rho family gtpase activating protein (GAP) required for central spindle formation and cytokinesis.
Academic Article A requirement for Rho and Cdc42 during cytokinesis in Xenopus embryos.
Academic Article Anaphase is initiated by proteolysis rather than by the inactivation of maturation-promoting factor.
Academic Article PLK1 plays dual roles in centralspindlin regulation during cytokinesis.
Academic Article Competition between kinesin-1 and myosin-V defines Drosophila posterior determination.
Academic Article Rho1 activation recapitulates early gastrulation events in the ventral, but not dorsal, epithelium of Drosophila embryos.
Academic Article Cortical recruitment of centralspindlin and RhoA effectors during meiosis I of Caenorhabditiselegans primary spermatocytes.
Academic Article Small GTPases modulate intrinsic and extrinsic forces that control epithelial folding in Drosophila embryos.
Academic Article Aurora A and cortical flows promote polarization and cytokinesis by inducing asymmetric ECT-2 accumulation.
Concept Aurora Kinase A
Concept Aurora Kinase B
Concept Aurora Kinases
Concept Anaphase-Promoting Complex-Cyclosome
Concept Apc3 Subunit, Anaphase-Promoting Complex-Cyclosome
Concept Apc6 Subunit, Anaphase-Promoting Complex-Cyclosome
Concept Rho Guanine Nucleotide Exchange Factors
Concept Separase
Concept Securin
Concept In Vitro Techniques
Academic Article Apical polarity and actomyosin dynamics control Kibra subcellular localization and function in Drosophila Hippo signaling.
Academic Article Projective light-sheet microscopy with flexible parameter selection.
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